Publications

Microscopic image showing a green elongated structure with scattered red dots and clusters on a black background.

Publications

* indicates co-first authors; ^ indicates co-corresponding authors

Time-course panels and quantifications show differentiation of human myoblasts, with comparisons of wildtype and CHAMP1 knockout, mouse transplantation schema, and histology of engrafted muscle featured in recent publications.

29. Zhang H., Zhou M., Zhang Z., Wang Z., Shi R., Wang Y., Wei X., Shang R., Li J., He C., Xie J., Diao Y., Bi P^. CHAMP1 is an essential regulator for human myoblast fusion and muscle development. Nature Communications 17:546 (2026).

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A scientific figure designed for publications, showing experimental workflow, cell marker distributions, gene expression heatmaps, and statistical data on gene distribution across cell clusters in a CRISPR knock-out study.

28. Zhang H., Shang R., Zhang Z., Zhou M., Bigot A., Cai Y., Zhao Y., Wang Y., Deshmukh A., Kudryashova E., Kudryashov DS., He C., Mouly V., Bi P^. Development of a split-toxin CRISPR screening platform to systematically identify regulators of human myoblast fusion. Nature Communications 17:547 (2026).

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Publication # 27

27. Bi P^, Geisbrecht ER^. Cell fusion: Inter-organ tissue communication promotes a union between myoblasts. Current Biology 34(9), R343–R345 (2024)

Publication # 26

26. Johnson CJ, Zhang Z, Zhang H, Shang R, Piekarz KM, Bi P, Stolfi A. A change in cis-regulatory logic underlying obligate versus facultative muscle multinucleation in chordates. Development 151(20), dev202968 (2024)

Publication 25-1

25. Zhang H, Shang R, Kim K, Zheng W, Johnson CJ, Sun L, Niu X, Liu L, Zhou J, Liu L, Zhang Z, Uyeno TA, Pei J, Fissette SD, Green SA, Samudra SP, Wen J, Zhang J, Eggenschwiler J, Menke D, Bronner ME, Grishin NV, Li W, Ye K, Zhang Y, Stolfi A^, Bi P^. Evolution of a chordate-specific mechanism for myoblast fusion. Science Advances. 2022 Sep 2;8(35):eadd2696.

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Diagram showing the evolution of myoblast fusion genes in chordates, gene duplication events, and the emergence of multinucleated myocytes, with related species and gene pathways labeled.

24. Zhang H, Shang R, Kim K, Zheng W, Johnson CJ, Sun L, Niu X, Liu L, Uyeno TA, Zhou J, Liu L, Pei J, Fissette SD, Green SA, Samudra SP, Wen J, Zhang J, Eggenschwiler J, Menke D, Bronner ME, Grishin NV, Li W, Ye K, Zhang Y, Stolfi A^, Bi P^. Evolution of a chordate-specific mechanism for myoblast fusion. bioRxiv. 24 July 2021. doi: https://doi.org/10.1101/2021.07.24.453587

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Figure with multiple panels showing gene editing and expression analysis of DLL1, including sequence alignment, ChIP-Seq data, statistical graphs, histology images, and mechanistic diagrams.

23. Zhang H, Shang R, Bi P^. Feedback regulation of Notch signaling and myogenesis connected by MyoD–Dll1 axis. PLoS Genetics. 2021 Aug 9;17(8):e1009729.

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Table summarizing gene information and four panels (B–E) showing CRISPR/Cas9 target sites and validation results for different genes with gel images and schematic diagrams.

22. Shang R, Zhang H, Bi P^. Generation of mouse conditional knockout alleles in one step using the i-GONAD method. Genome Research. 17 December 2020

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A scientific figure with graphs, microscopy images, diagrams, and charts showing experimental data comparing MyoX and MyoXI in human myotubes under different conditions.

21. Zhang H, Wen J, Bigot A, Chen J, Shang R, Mouly V, Bi P^. Human myotube formation is determined by MyoD–Myomixer/Myomaker axis. Science Advances. 2020; 6 : eabc4062 18 December 2020

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Bi Lab

20. Bi P, McAnally JR, Shelton JM, Sánchez-Ortiz E, Bassel-Duby R and Olson EN. The fusogenic micropeptide Myomixer is essential for satellite cell fusion and muscle regeneration. <PNAS. 2018; 115(15): 3864-3869.

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Bi Lab

19. Shi J*, Bi P*, Pei J, Li H, Grishin NV, Bassel-Duby R, Chen EH, Olson EN. Requirement of the fusogenic micropeptide myomixer for muscle formation in zebrafish. PNAS. 2017; 114(45): 11950-11955.

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Bi Lab

18. Bi P, Ramirez-Martinez A, Li H, Cannavino J, McAnally JR, Shelton JM, Sánchez-Ortiz E, Bassel-Duby R, Olson EN. Control of muscle formation by the fusogenic micropeptide myomixer. Science. 2017; 356(6335): 323-327.

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Press Coverage: Muscular Dystrophy News, ScienceDaily, MEMBS, F1000 Prime

Bi Lab

17. Bi P^, Yue F, Karki A, Castro B, Wirbisky SE, Wang C, Durkes A, Elzey BD, Andrisani OM, Bidwell CA, Freeman JL, Konieczny SF, Kuang S. Notch activation drives adipocyte dedifferentiation and tumorigenic transformation in mice. J Exp Med. 2016; 213(10):2019-37.

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Press Coverage: ScienceDaily, Medical Xpress, EurekAlert.

Bi Lab

16. Bi P*, Yue F*, Sato Y, Wirbisky S, Liu W, Shan T, Wen Y, Zhou D, Freeman J, Kuang S. Stage-specific effects of Notch activation during skeletal myogenesis. eLife. 2016; e17355.

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Bi

15. Bi P, Shan T, Liu W, Yue F, Yang X, Liang XR, Wang J, Li J, Carlesso N, Liu X, Kuang S. Inhibition of Notch signaling promotes browning of white adipose tissue and ameliorates obesity. Nat Med. 2014. 20(8):911-918.

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Highlighted in: Nature Medicine, Science Signaling.
Press Coverage: Innovations Report, ScienceDaily, HealthCanal, R&D.

Bi Lab - Trends Endocrinol Metab

14. Bi P^, Kuang S. Notch signaling as a novel regulator of metabolism. Trends Endocrinol Metab. 2015; 26(5): 248-55.

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Bi Lab - stem cell niche and postnatal muscle growth

13. Bi P, Kuang S. Meat Science and Muscle Biology Symposium: stem cell niche and postnatal muscle growth. J Anim Sci. 2012; 90(3):924-35.

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Bi Lab - miR-133a regulates adipocyte browning in vivo

12. Liu W*, Bi P*, Shan T, Yang X, Yin H, Wang YX, Liu N, Rudnicki MA, Kuang S. miR-133a regulates adipocyte browning in vivo. PLoS Genet. 2013; 9(7) :e1003626.

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11. Yue F, Bi P, Wang C, Li J, Liu X, Kuang S. Conditional loss of Pten in myogenic progenitors leads to postnatal skeletal muscle hypertrophy but age-dependent exhaustion of satellite cells. Cell Rep. 2016; 17(9): 2340-2353.

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10. Yue F, Bi P, Wang C, Shan T, Nie Y, Ratliff TL, Gavin TP, Kuang S. Pten is necessary for the quiescence and
maintenance of adult muscle stem cells. Nat Commun. 2017; 8:14328.

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9. Wen Y, Bi P, Liu W, Asakura A, Keller C, Kuang S. Constitutive Notch activation upregulates Pax7 and promotes the self-renewal of skeletal muscle satellite cells. Mol Cell Biol. 2012; 32(12):2300-11.

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8. Li Z, Li J, Bi P, Lu Y, Burcham G, Elzey BD, Ratliff T, Konieczny SF, Ahmad N, Kuang S, Liu X. Plk1 phosphorylation of PTEN causes a tumor-promoting metabolic state. Mol Cell Biol. 2014; 34(19):3642-61.

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7. Liu W, Wen Y, Bi P, Lai X, Liu XS, Liu X, Kuang S. Hypoxia promotes satellite cell self-renewal and enhances the efficiency of myoblast transplantation. Development. 2012; (16):2857-65.

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6. Yang X, Bi P, Kuang S. Fighting obesity: When muscle meets fat. Adipocyte. 2014; 3(4):280-9.

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5. Shan T, Liang X, Bi P, Kuang S. Myostatin knockout drives browning of white adipose tissue through activating the AMPK- PGC1α-Fndc5 pathway in muscle. FASEB J. 2013; 27(5): 1981-9.

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4. Shan T, Liang X, Bi P, Zhang P, Liu W, Kuang S. Distinct populations of adipogenic and myogenic Myf5-lineage progenitors in white adipose tissues. J Lipid Res. 2013; 54(8): 2214-24.

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3. Shan T, Zhang P, Bi P, Kuang S. Lkb1 deletion promotes ectopic lipid accumulation in muscle progenitor cells and mature muscles. J Cell Physiol. 2015; 230(5):1033-41.

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2. Shan T, Zhang P, Liang X, Bi P, Yue F, Kuang S. Lkb1 is indispensable for skeletal muscle development, regeneration, and satellite cell homeostasis. Stem Cells. 2014; 32(11): 2893-907.

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1. Shan T, Xiong Y, Zhang P, Li Z, Jiang Q, Bi P, Yue F, Yang G, Wang Y, Liu X, Kuang S. Lkb1 controls brown adipose tissue growth and thermogenesis by regulating the intracellular localization of CRTC3. Nat Commun. 2016; 7:12205.

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